WebHuman cytokines: intracellular staining quick guide. Annotations: Iono=Ionomycin; PMA=Phorbol Myristate Acetate; LPS=Lipopolysaccharide; 2d=2 day culture; 5hr=5 hour culture; LPS for activation of human PBMC obtained from Sigma (#L-8274) Web22 apr. 1999 · The level of CD69 expression on the cell surface of CD4 cells was used as a marker for successful inhibition of protein secretion. PMA/ionomycin stimulation increased the percentage of CD69-positive CD4 + cells from 2 to 78%. Monensin showed a concentration-dependent inhibition of protein secretion, but no complete block of protein …
Exosome-mediated extracellular release of polyadenylate
Web15 jun. 1992 · 1. Newly synthesized, intermediate, and mature forms in this case the differences between monensin and brefeldin o CgB and SgII. PC12 cells were pulse … Web26 dec. 2002 · The most widely used reagents are the antibiotics monensin and brefeldin A. The aim of this study was to evaluate and compare the efficiency of both monensin … cozumel all inclusive family resort day pass
Brefeldin A Solution 1,000X - BioLegend
Web29 jul. 2012 · Intracellular cytokine staining (ICS) assay optimization should include selection of suitable cytokine secretion inhibitors. Here, peripheral blood mononuclear cells (PBMC) from women with proven history of C. trachomatis genital tract infection were used to compare the ability of brefeldin A (BFA) and monensin (MN) to concurrently trap … WebBrefeldin A (BFA) Solution (RUO (GMP)) Protocol Library Scientific Resources Catalog No: Your Price: Sign In Unit: Size: 250 µL Product Details Description Intracellular protein transport inhibitor. Product Notices Please refer to www.bdbiosciences.com/us/s/resources for technical protocols. Data Sheets Safety Data Sheet 347688 Rev. 1 WebMoreover, monensin also augmented Golgi-derived tubules that contained the ER-Golgi-intermediate compartment marker, p58, in the absence of BFA, signifying the generality of this effect. Taken together, we propose that monensin inhibits membrane fusion processes in the presence or absence of BFA. Publication types Comparative Study magicow